解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科，口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学，全科和内科
医学，法律
医学，研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学，核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学，临床
abstract：:Transgenic mice were produced from DNA-injected embryos stored for 2 to 30 days in liquid nitrogen. Of the 500 zygotes collected from (C57BL/6 x CBA)F1 mice, 363 (73%) survived DNA injection into pronuclei and 246 (82%) morphologically normal 4- and 8-cell embryos were flushed from temporary recipients 48 h later. Of ...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:In medical practice aryloxycarbonic acid derivatives have been solely used in the treatment of hyperlipidemia. Recently we patented the use of some of these derivatives in the treatment of dermatological diseases. Further analyses showed that these substances stimulated various cells of mesodermal origin including lym...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:An alkylating agent, nitrogranulogen, enhances the in vitro production of interleukin 1 by human monocytes. On the other hand, the drug inhibits proliferative responses of mouse thymocytes to exogenously added IL-1. Our results may shed light on the mechanisms of anti-inflammatory action of immunosuppressive agents. ...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:In the natural population of Tetrix tenuicornis from the Kraków-Czestochowa Upland 26.7% of males showed various aberrations of the morphology and behaviour of chromosomes. The high frequency of chromosome aberrations found in this population indicates their genetic instability. ...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Plant DNA is often contaminated by chloroplast DNA. The contamination was used here with advantage to check restriction endonuclease cleavage of plant DNA. This method is based on hybridization of Southern blots of digested plant DNA with cloned fragments of chloroplast DNA as a probe. Advantages of this method are de...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Eight-cell embryos recovered from superovulated C57BL/6J mice were disaggregated into individual 1/8 blastomeres. For cryopreservation of blastomeres a simple "two-step" technique based on direct transfer of blastomeres into the final concentration of DMSO (0.5 to 1.5 M) and exposure of plastic straws with blastomeres...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The distribution of factor B (Bf) phenotypes and gene frequencies were investigated in 280 genetically unrelated persons of the Slovak population. Thin-layer agarose gel high-voltage electrophoresis and subsequent immunofixation were used. A low frequency of the "rare" allele BfFl was observed (BfFl = 0.0017). The fre...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:A "Quasi-dynamic Test of in vitro Cell Migration" (QTCM) was developed in which the quantitative estimation of migration of dividing cells is based on a comparative evaluation of their dispersion in single-cell-derived colonies after 72 h of clonal growth under standard and test conditions. Expert visual evaluation of...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The effect of a synthetic peptide, corresponding to a sequence of HIV-1 p24 protein (amino acids 218-237), on in vitro immune responses was studied. The peptide inhibited in a dose-dependent manner the induction of an anti-SRC antibody response and of a PPD-specific proliferative response of human PBL. On the other ha...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The purpose of the present study was to demonstrate visually and localize the presence of serotonin (5-HT) in the ovary and oviduct of the domestic hen using a histochemical Falck-Hillarp method. Experiments were carried out on White Leghorn laying hens with no egg in the shell gland. The specific yellow fluorescence,...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Protein kinase C from 10T1/2 cells can be eluted by linear gradient of NaCl in two fractions. Following treatment with 10(-5) M 13-cis-retinoic acid a decrease of total PKC activity was observed, mainly at the expense of the 150 mM NaCl eluted fraction. ...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Monoclonal antibodies against several human leucocyte cell surface antigens were prepared and characterized: (1) MEM-56, MEM-93, MEM-66, and MEM-104 against the CD45R antigen (220 kDa and 205 kDa mol. wt. forms of the leucocyte common antigen CD45 expressed on B-lymphocytes, myeloid cells and a subpopulation of T-lymp...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The possibility of expression of the gag gene of bovine leukaemia virus (BLV) in the bacterial system was investigated. The DNA fragment coding for the gag core 24 kDa protein of BLV was inserted into the pORF1 expression vector. The polypeptides expressed in E. coli were analysed by Western blotting. The bacterially ...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:A single intramuscular injection of kainic acid (12 micrograms/g body weight, dissolved in 0.1 ml of aqua pro injectione) was administered to adult, sexually mature, male mice. Control mice received equivalent amounts of aqua pro injectione only. Experimental animals were killed 24, 72, 120, and 192 h after the inject...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Sexually mature male and female frogs, Rana temporaria L., taken from their natural habitat at the end of the hibernation period, were subjected to treatment with ultrasound of various duration. The ultrasound (frequency, 24 kHz, intensity 0.35 W/cm2) was applied over the whole body in a suitable water bath. Neurons o...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Age-dependent decline of the anti-tumour efficacy of local IL-2 immunotherapy and chemoimmunotherapy utilizing human recombinant interleukin 2 and cyclophosphamide was investigated in B10 mice bearing syngeneic MC-induced sarcoma. Repeated peritumoral injections of rIL-2 substantially inhibited growth of transplantabl...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Histochemical methods were used to study the localization and activity of acid and alkaline phosphatases, ATP-ase, 5-nucleotidase, and glucose-6-phosphatase in tissues of the mature form of Hymenolepis nana. Considerable differences in activity and localization of particular enzymes were observed in the organs of the ...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The suppressive effect of xenogeneic blood transfusion on T-cell reactivity, observed in both in vivo and in vitro studies, is connected with the development of indomethacin-sensitive suppressor cells. ...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Japanese quail oviduct total cDNA was synthesized from total mRNA by the classical method, using AMV reverse transcriptase, the Klenow fragment of DNA polymerase I and S1 nuclease. The results of the synthesis of total ss-cDNA partially differed from those published for the synthesis of chicken total ss-cDNA. The pres...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The genome structure of a new avian src-containing acute transforming virus, designated PR2257, was studied by restriction analysis. PR2257 was isolated from a sarcoma grown in a 155-day-old chick inoculated intraembryonally with a transformation-defective mutant of daPR-C retaining no src sequences. The 4.4-kb genome...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:A new cell line, GS, of human renal cell carcinoma was established and characterized. It was derived from a metastasis of a human renal cell carcinoma, which appeared 6 years after nephrectomy. The GS cells exhibit basic characteristics of renal cell carcinoma: epithelial cell character, PAS and glycogen positivity, t...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:On the basis of karyotype analysis of the 6 groups of mouse--mouse hybridomas secreting monoclonal antibodies against 5 different antigens we found: (1) The karyotypic variability of clones in all six compared groups of hybridomas was consistent and was influenced neither by the specificity of secreted monoclonal anti...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:At the exponential growth phase, nystatin-treated Saccharomyces cerevisiae cells stain with Rhodamine B if they have not previously been exposed to killer toxin produced by Kluyveromyces lactis. This finding constitutes the principle of a method for estimating the activity of this toxin. ...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:The disturbances of the arachidonic acid metabolic cascade are supposed to play a significant part in some membrane alterations and in associated immunopathological changes in multiple sclerosis. Since the lipoxygenase derivatives of the arachidonic acid and the calcium ion influx were proved to participate in the mec...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Tolerogenic treatment of mice by successive injections of lipopolysaccharide (LPS) from E. coli or S. marcescens and cyclophosphamide (CY) decreased both the specific and polyclonal responses to tolerogen and to irrelevant LPS from Br. abortus as well as the specific immune response to sheep red blood cells. Splenocyt...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Peripheral blood mononuclear cells from young adult and aged human donors were activated with PHA and the expression of IL-2 receptors was examined. For the detection of IL-2 receptor-positive cells, flow cytofluorometry using monoclonal antibody Clonab IL-2R, fluorescein-conjugated swine anti-mouse IgG and fluorescen...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:A comparative histoenzymological study of the distribution of Ca++-activated adenosine triphosphatase amongst the various constituents of the retinae of Varanus and Ptyas, hitherto not reported in the literature, has been made in great detail. The outer segment has shown an enzymatic activity of a very high degree bot...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:A transformation-defective mutant of Rous sarcoma virus (td PR-RSV-C) inoculated into chicken embryos caused a number of pathological changes in chickens after hatching. Disturbances in haematopoiesis were manifested by anaemia of different degree and by the presence of immature blood elements in the peripheral blood....
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:A simple method of revealing proteins on nitrocellulose blots is described. It is based on high affinity of colloidal silver for proteins. The sensitivity is about the same as that of colloidal gold and it appears to be about 20 times higher than the presently used staining methods. The whole procedure is very simple ...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Hybridomas secreting anti-HBsAg antibodies were produced by fusion of the mouse myeloma cell line SP2/0 with lymphocytes from mice immunized with purified HBsAg. All clones produced antibodies of the IgG1 idiotype that react with the subtype a determinant of HBsAg. An enzyme immunoassay for detection of HBsAg in human...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Among the 7 endogenous proviruses we have detected in Brown Leghorn chickens none encodes the production of virions and only one, ev-3, expresses the gag gene. To study the possible role of DNA methylation in the inhibition of provirus expression, we performed blot hybridization and restriction endonuclease analysis w...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:A monoclonal antibody, VI-01, to vimentin and desmin has been isolated from a fusion of mouse myeloma cells with immune spleen cells. The antibody appeared specific for vimentin of various species and smooth muscle desmin and did not recognize other intermediate filament proteins when cell lines and tissue sections we...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Ampicillin was found to have an immunoenhancing effect on the expression of FcM human lymphocyte receptors, on the angiogenic potential of human lymphocytes and on the production of anti-SRBC antibody after in vivo administration to low-responder strains of mice. The effect of ampicillin on the graft-versus-host disea...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Computer comparisons of amino acid sequences from 8 retroviral oncogenes and 26 protein hormones were done with respect to structure similarity and so-called uninterrupted structure similarity. Sequence homology was found between v-myc, the transforming protein of MC29 virus, and human gastrin and oxytocin on the one ...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Natural antibodies were detected in normal sera of diabetes-prone rats of substrain dp BB/DK (19.5%), non-diabetes-prone ndp BB/PhiK (17.8%) and their ancestral randombred strain WOK (2.2-7.5%). These antibodies were haemagglutinins specifically reacting with antigenic products of the RT1.A region, mainly with product...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Selectivity of mouse monoclonal antibody 7E9 (IGG3) directed against human urinary bladder carcinoma cells has been examined by indirect membrane immunofluorescence, using a panel of 31 human cell lines. The 7E9 monoclonal antibody discriminated between urinary bladder carcinoma cells and normal urothelium or cells of...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Four hybridomas making monoclonal antibodies against horseradish peroxidase have been established. Two of them are IgM and two are IgG1 with kappa light chains. Antibody HP-03 has been selected as the most suitable one for preparation of the peroxidase-anti-peroxidase complex. Furthermore, optimal conditions for const...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Two types of changes in the spin-spin relaxation time of water protons (T2) corresponding to the decrease in the correlation time of water molecules have been found in human serum albumin solutions in the temperature range of 20-40 degrees using the NMR method. It follows from experiments with frozen HSA solutions tha...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:The possibility was tested whether tolerance can be induced in newly hatched chickens by a single injection of HSA on the day of hatching in doses one or several orders lower than the dose employed in previous experiments (100 mg of HSA). Doses of 10 and 1 mg of HSA induced a statistically significant suppression of a...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:The effect of lentil lectin on the survival of skin allografts was investigated in six rat strain combinations with different genetic disparities between the donors and recipients. LCA was administered i.p. (50 mg/kg) to the recipients daily, starting with the day of transplantation (day 0) until graft rejection, exce...
journal_title：Folia biologica
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
